ABSTRACT
The present disclosure relates to acetaminophen protein adducts and methods of diagnosing acetaminophen toxicity using the acetaminophen protein adducts.
CLAIMS
1. A method for measuring the amount of acetaminophen-protein adduct in a biological sample, the method comprising: a) obtaining a biological sample from a subject having or suspected of having acetaminophen toxicity; and b) measuring the total amount of acetaminophen-protein adduct in the sample, wherein the acetaminophen-protein adducts are detected using high performance liquid chromatography (HPLC) or an immunoassay comprising an antibody with specific for NAPQI bound to a protein through a 3-(cystein-S-yl) APAP (3-Cys-A)-protein linkage.