ABSTRACT
The present disclosure provides isolated antibodies that bind to acetaminophen-protein adducts that are useful in the detection and diagnosis of acetaminophen-induced toxicity.
CLAIMS
1. An immunoassay comprising at least one isolated antibody which specifically binds an acetaminophen-protein adduct but does not specifically bind free acetaminophen, wherein the antibody comprises: (a) a light chain CDR1 amino acid sequence of SEQ ID NO:21; (b) a light chain CDR2 amino acid sequence of SEQ ID NO:22; (c) a light chain CDR3 amino acid sequence of LGh; wherein h is a hydrophobic amino acid; (d) a heavy chain CDR1 amino acid sequence of YXI, wherein X is any amino acid; (e) a heavy chain CDR2 amino acid sequence of SEQ ID NO:23; and (f) a heavy chain CDR3 amino acid sequence of SEQ ID NO:24.